Innovative fluorescent probes for in vivo visualization of biomolecules in living <i>Caenorhabditis elegans</i>

Caenorhabditis elegans (C. elegans) as a well-established multicellular model organism has been widely used in the biological field for half century. Its numerous advantages including small body size, rapid life cycle, high-reproductive rate, well-defined anatomy, and conserved genome, made C. one of most successful organisms. Discoveries obtained from have great contributions to research fields such development, aging, biophysics, immunology, neuroscience. Because its transparent giant cell is also an ideal subject high resolution high-throughput optical imaging analysis. During past decade, advances develop biomolecule-targeting techniques noninvasive imaging. These novel technologies expanded toolbox qualitative quantitative analysis biomolecules elegans. In this review, we summarize recently developed fluorescent probes or labeling visualizing at cellular, subcellular molecular scale by using major designed specifically applications Combining technological with will provide new horizons high-efficiency live